Bead Method for the Detection of Sperm-Reactive IgM Antibodies
- 100 determinations per reagent recommended as a replacement to the ImmunoBeadsTM Test
- (IBT) improvement over IBT-- see comparison below
- 18-month shelf life
There is considerable evidence that antibodies directed against sperm surface antigens exert a fertility-reducing effect. When antisperm antibodies are present, they bind to the surface of the sperm. Such sperm-bound antibodies may impair sperm movement, penetration of the cumulus and/or zona pellucida, the acrosome reaction, interaction of sperm and egg as well as identify the sperm for destruction by the immune system. It has also been suggested that sperm antibodies may exert an embryotoxic effect. Finally, there is evidence that antibody concentration is correlated directly with the severity of sperm functional impairment and inversely with potential fertilizability.
For these reasons, many reproductive specialists screen sperm and/or serum for anti-sperm antibodies before choosing an appropriate Assisted Reproductive Technology.
Comparison of ImmunoSpheres® and ImmunoBeadsTM (IBT)
ImmunoSpheres and ImmunoBeads mixed on a slide, 400X.
Courtesy of Grace M. Centola.
|Beads||monodispersed||tend to clump|
|Bead Size||uniform 3.0um latex beads||non-uniform size beads; can be mistaken for cellular debris|
|Bead Color||blue for Anti-IgG
blue for Anti-IgA
blue for Anti-IgM
white for Anti-Ig(H&L)
|Bead Preparation||wash each time||weigh out and wash each time|
|Microscopy||bright field or phase contrast||phase contrast|
|Shelf Life||18 months||1 month once reconstituted|